Evaluación de las propiedades mecánicas de hidrogeles a base de agarosa, funcionalizados con plasma pobre en plaquetas con potencial uso para la cicatrización de úlceras por presión

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dc.contributor.advisorBecerra Bayona, Silvia Milena
dc.contributor.advisorSolarte David, Víctor Alfonso
dc.contributor.apolounabBecerra Bayona, Silvia Milena [silvia-milena-becerra-bayona]
dc.contributor.authorAcevedo Mendoza, Anderson
dc.contributor.authorSalazar Hernández, Iván Stevens
dc.contributor.authorSilva García, Mariana
dc.contributor.cvlacBecerra Bayona, Silvia Milena [0001568861]spa
dc.contributor.cvlacSolarte David, Víctor Alfonso [0001329391]spa
dc.contributor.googlescholarBecerra Bayona, Silvia Milena [5wr21EQAAAAJ]spa
dc.contributor.linkedinBecerra Bayona, Silvia Milena [silvia-becerra-3174455a]
dc.contributor.orcidBecerra Bayona, Silvia Milena [0000-0002-4499-5885]spa
dc.contributor.orcidSolarte David, Víctor Alfonso [0000-0002-9856-1484]spa
dc.contributor.researchgateBecerra Bayona, Silvia Milena [Silvia_Becerra-Bayona]spa
dc.contributor.scopusBecerra Bayona, Silvia Milena [36522328100]spa
dc.contributor.scopusBecerra Bayona, Silvia Milena [36522328100]
dc.coverage.campusUNAB Campus Bucaramangaspa
dc.coverage.spatialColombiaspa
dc.date.accessioned2022-07-11T16:43:40Z
dc.date.available2022-07-11T16:43:40Z
dc.date.issued2022
dc.degree.nameIngeniero Biomédicospa
dc.description.abstractLas úlceras por presión (UPP) se producen debido a una presión o cizallamiento constante y excesivo en la piel o tejidos que la componen, lo cual, produce una falta de irrigación que conlleva a lesiones cutáneas que pueden originar infecciones, irritación e incluso necrosis. Los tratamientos actuales como los apósitos convencionales o avanzados no cumplen con todos los requisitos para permitir la regeneración del tejido, exhibiendo la necesidad de elaborar un apósito apto para proporcionar un ambiente adecuado para la curación de la herida. Por consiguiente, en este proyecto se fabricaron hidrogeles de agarosa funcionalizados con plasma pobre en plaquetas (PPP), debido a que contiene proteínas que desempeñan un papel importante durante las etapas de hemostasia y coagulación, promoviendo la reparación del tejido epitelial y la cicatrización de heridas. Inicialmente, los hidrogeles se fabricaron usando tres concentraciones de agarosa (1, 1.5 y 2%), y tres concentraciones de PPP (25, 50 y 75%); y para cada formulación se realizó su respectiva muestra control. Las propiedades mecánicas como módulo de elasticidad y compresión se evaluaron mediante pruebas de tensión y compresión, mientras que la dinámica de liberación de proteínas se determinó mediante la cuantificación de proteínas usando una curva de estándar de BSA. Adicionalmente, para determinar la viabilidad celular de los hidrogeles fabricados, se realizó un ensayo preliminar de citotoxicidad con células HT1080. Como resultados, el módulo de elasticidad de los hidrogeles de agarosa con PPP osciló entre 0.0084 MPa y 0.0203 MPa, mientras que el módulo de compresión varió entre 0.0628 MPa y 0.2168 MPa. La dinámica de liberación de proteínas determinó que, a mayores concentraciones de PPP en los hidrogeles fabricados, incrementaba la concentración de proteínas presentes en estos. Finalmente, los resultados del ensayo preliminar de citotoxicidad sugirieron que los hidrogeles de agarosa funcionalizados con PPP que se fabricaron bajo la presente metodología, contribuyen de forma limitada con la viabilidad celular.spa
dc.description.abstractenglishPressure ulcers (UPP) occur due to constant and excessive pressure or shear on the skin or tissues that compose it, which produces a lack of irrigation that leads to skin lesions that can cause infections, irritation and even necrosis. Current treatments such as conventional or advanced dressings do not meet all the requirements to allow tissue regeneration, exhibiting the need to elaborate a dressing suitable to provide a suitable environment for wound healing. Therefore, in this project, functionalized agarose hydrogels were manufactured with platelet-poor plasma (PPP), because it contains proteins that play an important role during the hemostasis and coagulation stages, promoting the repair of epithelial tissue and wound healing. Initially, the hydrogels were manufactured using three concentrations of agarose (1, 1.5 and 2%), and three concentrations of PPP (25, 50 and 75%); and for each formulation its respective control sample was performed. Mechanical properties such as modulus of elasticity and compression were assessed by stress and compression tests, while protein release dynamics were determined by quantifying proteins using a BSA standard curve. Additionally, to determine the cell viability of the manufactured hydrogels, a preliminary cytotoxicity test was performed with HT1080 cells. As a result, the modulus of elasticity of agarose hydrogels with PPP ranged from 0.0084 MPa to 0.0203 MPa, while the compression modulus ranged from 0.0628 MPa to 0.2168 MPa. The dynamics of protein release determined that, at higher concentrations of PPP in the manufactured hydrogels, the concentration of proteins present in them increased. Finally, the results of the preliminary cytotoxicity test suggested that PPP-functionalized agarose hydrogels manufactured under the present methodology contribute to cell viability in a limited way.spa
dc.description.degreelevelPregradospa
dc.description.learningmodalityModalidad Presencialspa
dc.description.tableofcontentsCapítulo 1. Problema u oportunidad ............................................................................................... 9 1.1 Planteamiento del problema ................................................................................................... 9 1.2 Justificación ........................................................................................................................... 11 1.3 Pregunta problema ................................................................................................................ 12 1.4 Objetivo general .................................................................................................................... 13 1.5 Objetivos específicos ............................................................................................................. 13 Capítulo 2. Marco teórico ............................................................................................................... 14 2.1 La piel y sus propiedades mecánicas ................................................................................... 14 2.2 Úlceras por presión (UPP) .................................................................................................... 16 2.2.1 Características fisiopatológicas de las UPP .................................................................. 16 2.2.2 Proceso de cicatrización de una UPP ............................................................................ 17 2.2.3 Prevenciones y tratamientos de las UPP ...................................................................... 19 2.3 Hidrogeles de agarosa ........................................................................................................... 21 2.4 Factores de Crecimiento ....................................................................................................... 25 2.4.1 Plasma rico en plaquetas (PRP) .................................................................................... 25 2.4.2 Plasma pobre en plaquetas (PPP) ................................................................................. 26 2.5 Formación de hidrogeles de fibrina ..................................................................................... 27 Capítulo 3. Estado del arte ............................................................................................................. 30 Capítulo 4. Metodología .................................................................................................................. 33 4.1 Fabricación de hidrogeles de agarosa funcionalizados con PPP ....................................... 33 4.2 Dinámica de liberación de proteínas ................................................................................... 36 4.3 Caracterización mecánica de los hidrogeles ....................................................................... 38 4.4 Ensayo preliminar de Citotoxicidad .................................................................................... 41 4.5 Análisis estadísticos ............................................................................................................... 43 Capítulo 5. Resultados y Análisis de Resultados .......................................................................... 44 5.1 Resultados .............................................................................................................................. 44 5.1.1 Fabricación de hidrogeles a base de agarosa funcionalizados con PPP .................... 44 5.1.2 Caracterización mecánica de los hidrogeles ................................................................ 47 5.1.3. Dinámica de liberación de proteínas ........................................................................... 61 5.1.4. Ensayo preliminar de citotoxicidad ............................................................................. 70 5.2 Análisis de resultados ............................................................................................................ 72 Capítulo 6. Conclusiones y Recomendaciones .............................................................................. 79 Listado de Referencias .................................................................................................................... 81 Anexos .............................................................................................................................................. 88spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameinstname:Universidad Autónoma de Bucaramanga - UNABspa
dc.identifier.reponamereponame:Repositorio Institucional UNABspa
dc.identifier.repourlrepourl:https://repository.unab.edu.cospa
dc.identifier.urihttp://hdl.handle.net/20.500.12749/16884
dc.language.isospaspa
dc.publisher.facultyFacultad Ingenieríaspa
dc.publisher.grantorUniversidad Autónoma de Bucaramanga UNABspa
dc.publisher.programPregrado Ingeniería Biomédicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
dc.rights.localAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/*
dc.subject.keywordsBiomedical engineeringspa
dc.subject.keywordsEngineeringspa
dc.subject.keywordsMedical electronicsspa
dc.subject.keywordsBiological physicsspa
dc.subject.keywordsBioengineeringspa
dc.subject.keywordsMedical instruments and apparatusspa
dc.subject.keywordsMedicinespa
dc.subject.keywordsBiomedicalspa
dc.subject.keywordsClinical engineeringspa
dc.subject.keywordsHydrogelsspa
dc.subject.keywordsAgarosespa
dc.subject.keywordsPressure ulcersspa
dc.subject.keywordsPlatelet poor plasmaspa
dc.subject.keywordsMechanical propertiesspa
dc.subject.keywordsProtein releasespa
dc.subject.keywordsBlood proteinsspa
dc.subject.keywordsBlood plasmaspa
dc.subject.keywordsMechanical propertiesspa
dc.subject.keywordsPolymersspa
dc.subject.lembIngeniería biomédicaspa
dc.subject.lembIngenieríaspa
dc.subject.lembBiofísicaspa
dc.subject.lembBioingenieríaspa
dc.subject.lembMedicinaspa
dc.subject.lembBiomédicaspa
dc.subject.lembProteínas de la sangrespa
dc.subject.lembPlasma sanguíneospa
dc.subject.lembPropiedades mecánicasspa
dc.subject.lembPolímerosspa
dc.subject.proposalIngeniería clínicaspa
dc.subject.proposalElectrónica médicaspa
dc.subject.proposalInstrumentos y aparatos médicosspa
dc.subject.proposalHidrogelesspa
dc.subject.proposalAgarosaspa
dc.subject.proposalUlceras por presiónspa
dc.subject.proposalPlasma pobre en plaquetasspa
dc.subject.proposalPropiedades mecánicasspa
dc.subject.proposalLiberación de proteínasspa
dc.titleEvaluación de las propiedades mecánicas de hidrogeles a base de agarosa, funcionalizados con plasma pobre en plaquetas con potencial uso para la cicatrización de úlceras por presiónspa
dc.title.translatedEvaluation of the mechanical properties of agarose-based hydrogels, functionalized with platelet-poor plasma with potential use for the healing of pressure ulcersspa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTrabajo de Gradospa
dc.type.redcolhttp://purl.org/redcol/resource_type/TP

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