Comparación de condiciones de cultivo empleadas para la expansión de células madre mesenquimales obtenidas a partir de medula ósea y tejido adiposo

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dc.contributor.authorBarajas, Juan
dc.contributor.authorArias, Anderson
dc.contributor.authorDíaz, Víctor
dc.contributor.authorBecerra, Silvia
dc.contributor.authorSolarte, Víctor
dc.contributor.researchgroupSemilleros de Investigación UNABspa
dc.coverage.campusUNAB Campus Bucaramangaspa
dc.coverage.spatialBucaramanga (Santander, Colombia)spa
dc.coverage.temporal2018spa
dc.date.accessioned2023-08-26T13:22:26Z
dc.date.available2023-08-26T13:22:26Z
dc.date.issued2018-11
dc.description.abstractEn la última década, el uso de células madres mesenquimales (MSCs) ha sido considerado una alternativa segura y efectiva en una amplia gama de patologías. Sin embargo, uno de los problemas que enfrenta la producción de MSCs a escala clínica, es el uso de componentes xenogénicos para su expansión in vitro. La presente propuesta de investigación busca comparar los resultados obtenidos por diferentes investigadores en relación a la eficacia del cultivo de las MSCs en suero fetal bovino y plasma rico en plaquetas. Esta comparación se llevará a cabo mediante una revisión sistemática usando los estudios reportados en diversas bases de datos hasta la fecha. Con esta investigación se espera discernir los pros y contras del uso de factores xenogénicos versus el plasma rico en plaquetas en la expansión de las MSCs.spa
dc.description.abstractenglishIn the last years, the use of mesenchymal stem cells (MSCs) has been considered a safe and effective alternative in a number of pathologies. However, the use of xenogeneic components in the expansion of MSCs in vitro, is one of the problems that MSC clinical production is facing. This research proposal has the goal of comparing the MSC culture efficacy in fetal bovine serumand platelet rich plasma. The data will be taken from results reported by different researchers that are published in databases up to date. As a result, a systematic review will be performed. We hope to gain some insight into the pros and cons of using xenogeneic factors vs. platelet rich plasma in MSC expansion.spa
dc.description.learningmodalityModalidad Presencialspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameinstname:Universidad Autónoma de Bucaramanga - UNABspa
dc.identifier.issnISSN 2344-7079spa
dc.identifier.reponamereponame:Repositorio Institucional UNABspa
dc.identifier.repourlrepourl:https://repository.unab.edu.cospa
dc.identifier.urihttp://hdl.handle.net/20.500.12749/21415
dc.language.isospaspa
dc.publisher.deparmentSistema de Investigación SIUNABspa
dc.publisher.facultyFacultad Ciencias de la Salud
dc.publisher.grantorUniversidad Autónoma de Bucaramanga UNABspa
dc.publisher.programPregrado Medicina
dc.relation.ispartofseriesGeneración Creativa : Encuentro de Semilleros de Investigación UNABspa
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dc.relation.referencesCaplan, A. I. (2015). Adult mesenchymal stem cells: When, where, and how. Stem Cells International.spa
dc.relation.referencesBieback, K., et al. (2009). Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells, 27(9), 2331-2341.spa
dc.relation.referencesGore, A. V., et al. (2015). Mesenchymal stem cells reverse bone marrow dysfunction following injury and stress. The Journal of Trauma and Acutespa
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dc.relation.referencesBorrione, P., Gianfrancesco, A. D., Pereira, M. T., and Pigozzi, F. (2010). Platelet-rich plasma in muscle healing. American Journal of Physical Medicine & Rehabilitation, 89(10), 854- 861.spa
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dc.relation.referencesMuller I, et al. (2006). Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy. 8(5):437-44.spa
dc.relation.referencesChieregato K, et al. (2011). Epidermal growth factor, basic fibroblast growth factor and platelet-derived growth factorbb can substitute for fetal bovine serum and compete with human platelet-rich plasma in the ex vivo expansion of mesenchymal stromal cells derived from adipose tissue. Cytotherapy.13(8):933-43.spa
dc.relation.referencesMojica-Henshaw, et al. (2013). Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures. Cytotherapy. 15(12):1458-68.spa
dc.relation.referencesAstori G, et al.(2016). Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future. Stem Cell Res Ther. 7(1):93.spa
dc.relation.referencesGoodarzi, P. et al. (2014). Human autologous serum as a substitute for fetal bovine serum in human Schwann cell culture. Acta Med Iran. 52(4):241-5.spa
dc.relation.referencesDessels, C. et al. (2016). Making the Switch: Alternatives to Fetal Bovine Serum for Adipose-Derived Stromal Cell Expansion. Front Cell Dev Biol. 4:115.spa
dc.relation.referencesHarrison, P. and Cramer, EM.(1993). Platelet alpha-granules. Blood Rev. 7(1):52-62.spa
dc.relation.referencesFekete, N. et al. (2012). Platelet lysate from wholeblood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components. Cytotherapy. 14(5):540-54spa
dc.relation.urihttp://hdl.handle.net/20.500.12749/14242
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
dc.rights.localAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/*
dc.sourceBarajas, J., et al. (2018). Comparación de condiciones de cultivo empleadas para la expansión de células madre mesenquimales obtenidas a partir de medula ósea y tejido adiposo. Recuperado de: http://hdl.handle.net/20.500.12749/21415spa
dc.subject.keywordsHealth sciencesspa
dc.subject.keywordsBasic sciencesspa
dc.subject.keywordsBone marrowspa
dc.subject.keywordsInvestigationspa
dc.subject.keywordsMSC'sspa
dc.subject.keywordsPlasmaspa
dc.subject.keywordsFetal bovine serumspa
dc.subject.keywordsAdipose tissuespa
dc.subject.lembCiencias de la saludspa
dc.subject.lembCiencias básicasspa
dc.subject.lembMédula óseaspa
dc.subject.lembInvestigaciónspa
dc.subject.proposalMSCsspa
dc.subject.proposalPlasmaspa
dc.subject.proposalSuero fetal bovinospa
dc.subject.proposalTejido adiposospa
dc.titleComparación de condiciones de cultivo empleadas para la expansión de células madre mesenquimales obtenidas a partir de medula ósea y tejido adiposospa
dc.title.translatedComparison of culture conditions used for the expansion of mesenchymal stem cells obtained from from bone marrow and adipose tissuespa
dc.typeConferenceeng
dc.type.coarhttp://purl.org/coar/resource_type/c_f744
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.driverinfo:eu-repo/semantics/conferenceProceedingsspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localMemoria de eventosspa
dc.type.redcolhttp://purl.org/redcol/resource_type/EC_AC

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