Estudio comparativo del consumo crónico de vino tinto sobre la expresión y la actividad del citocromo P450 en hígado y riñón de rata

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dc.contributor.authorHuerta Bustamante, Patricio Andrésspa
dc.contributor.authorHenríquez Huerta, Patricio Alejandrospa
dc.contributor.authorCastillo Peñaloza, Rodrigo Luisspa
dc.contributor.authorCarrasco Loza, Rodrigo Andresspa
dc.contributor.authorOrellana, Myriamspa
dc.contributor.authorRodrigo Salinas, Ramón Aníbalspa
dc.date.accessioned2020-10-27T14:22:17Z
dc.date.available2020-10-27T14:22:17Z
dc.date.issued2003-05-09
dc.description.abstractEl metabolismo del etanol involucra la participación de isoenzimas del citocromo P450 (CYP), principalmente con la contribución de la isoenzima 2E1 (CYP 2E1), cuya actividad es inducida por la exposición crónica al alcohol. El metabolismo etílico está relacionado con la producción de especies reactivas de oxígeno (ROS), responsables de la generación de estrés oxidativo. Por otra parte, se ha sugerido que los antioxidantes contenidos en el vino ejercerían un efecto protector contra la injuria oxidativa. El objetivo de este estudio fue comparar el efecto del consumo moderado de vino tinto sobre la expresión y la actividad del CYP 2E1 en hígado y riñón de rata. Ratas macho adultas fueron tratadas con agua (control), etanol (12,5%), vino tinto (12,5% de etanol) o vino tinto desalcoholizado por 10 semanas. El contenido de CYP total y CYP 2E1 fue evaluado en la fracción microsomal de riñón e hígado. La oxidación del etanol y p-nitrofenol fue tomada como índice de actividad de CYP 2E1, analizándose la contribución relativa de etanol y componentes no alcohólicos del vino. El etanol aumentó los contenidos de CYP total y CYP 2E1, así como la hidroxilación del p-nitrofenol y la oxidación del etanol tanto en hígado como en riñón. Esos efectos fueron atenuados por la administración de vino tinto. Con esta información es posible sugerir que los componentes no alcohólicos del vino tinto son capaces de modular el aumento en la expresión y la actividad del CYP 2E1 de hígado y riñón de la rata inducida por etanol. [Huerta PA, Henríquez PA, Castillo RL, Carrasco RA, Orellana M, Rodrigo RA. Estudio comparativo del consumo crónico de vino tinto sobre la expresión y la actividad del citocromo P450 en hígado y riñón de rata. MEDUNAB 2003spa
dc.description.abstractenglishEthanol metabolism involves the participation of cytochrome P450 (CYP) isoenzymes, mainly with the contribution of isoenzyme 2E1 (CYP 2E1), whose activity is induced by chronic alcohol exposure. Ethyl metabolism is related to the production of reactive oxygen species (ROS), responsible for the generation of oxidative stress. On the other hand, it has been suggested that the antioxidants contained in wine would exert a protective effect against oxidative injury. The objective of this study was to compare the effect of moderate red wine consumption on the expression and activity of CYP 2E1 in rat liver and kidney. Adult male rats were treated with water (control), ethanol (12.5%), red wine (12.5% ​​ethanol), or dealcoholized red wine for 10 weeks. The content of total CYP and CYP 2E1 was evaluated in the microsomal fraction of kidney and liver. The oxidation of ethanol and p-nitrophenol was taken as an index of CYP 2E1 activity, analyzing the relative contribution of ethanol and non-alcoholic components of the wine. Ethanol increased the contents of total CYP and CYP 2E1, as well as p-nitrophenol hydroxylation and ethanol oxidation in both liver and kidney. These effects were attenuated by the administration of red wine. With this information it is possible to suggest that the non-alcoholic components of red wine are capable of modulating the increase in the expression and activity of CYP 2E1 in the liver and kidney of the rat induced by ethanol. [Huerta PA, Henríquez PA, Castillo RL, Carrasco RA, Orellana M, Rodrigo RA. Comparative study of chronic red wine consumption on the expression and activity of cytochrome P450 in rat liver and kidney. MEDUNAB 2003eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameinstname:Universidad Autónoma de Bucaramanga UNABspa
dc.identifier.issn2382-4603
dc.identifier.issn0123-7047
dc.identifier.repourlrepourl:https://repository.unab.edu.co
dc.identifier.urihttp://hdl.handle.net/20.500.12749/10519
dc.language.isospaspa
dc.publisherUniversidad Autónoma de Bucaramanga UNAB
dc.publisher.facultyFacultad Ciencias de la Saludspa
dc.publisher.programPregrado Medicinaspa
dc.relationhttps://revistas.unab.edu.co/index.php/medunab/article/view/263/246
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dc.relation.urihttps://revistas.unab.edu.co/index.php/medunab/article/view/263
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 2.5 Colombia*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.sourceMedUNAB; Vol. 6 Núm. 16 (2003): Efectos del vino tinto, Ciclo celular, Hemofilia B, Endometriosis y dolor, Diagnóstico de tuberculosis; 4-9
dc.subjectCiencias biomédicas
dc.subjectCiencias de la vida
dc.subjectInnovaciones en salud
dc.subjectInvestigaciones
dc.subject.keywordsHealth Scienceseng
dc.subject.keywordsMedicineeng
dc.subject.keywordsMedical Scienceseng
dc.subject.keywordsBiomedical Scienceseng
dc.subject.keywordsLife Scienceseng
dc.subject.keywordsInnovations in healtheng
dc.subject.keywordsResearcheng
dc.subject.keywordsCytochrome P450eng
dc.subject.keywordsEthanoleng
dc.subject.keywordsOxidative stresseng
dc.subject.keywordsRat kidneyeng
dc.subject.keywordsRat livereng
dc.subject.lembCiencias médicasspa
dc.subject.lembCiencias de la saludspa
dc.subject.lembMedicinaspa
dc.subject.proposalCitocromo P450spa
dc.subject.proposalEtanolspa
dc.subject.proposalEstrés oxidativospa
dc.subject.proposalRiñón de la rataspa
dc.subject.proposalHígado de la rataspa
dc.titleEstudio comparativo del consumo crónico de vino tinto sobre la expresión y la actividad del citocromo P450 en hígado y riñón de rataspa
dc.title.translatedComparative study of chronic red wine consumption on the expression and activity of cytochrome P450 in rat liver and kidneyeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversionInfo:eu-repo/semantics/publishedVersion
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa
dc.type.redcolhttp://purl.org/redcol/resource_type/ART

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